PEPTIDE EVALUATION OF PROTEIN HYDROLYSATE FROM TILAPIA WASTE: FRACTIONATION, COMPOSITION, AND FEASIBILITY OF USE IN FISH DIETS
DOI:
https://doi.org/10.63330/aurumpub.012-045Keywords:
Peptides, SDS-PAGE, TilapiaAbstract
Aquaculture currently requires a sustainable, viable ingredient that can compete nutritionally with fishmeal. The objectives of this study were to produce, evaluate, and characterize silages from Nile tilapia filleting residues. The experiment was conducted in a completely randomized design with twelve treatments in a 2x2x3 factorial arrangement with three replicates. Two types of raw material (viscera or total residues: head, spine, fins, and viscera), two hydrolysis processes (acid and fermented), and three process interruption methodologies (heating, freezing, or neutralization) were used. Samples of each silage, from the different types of raw materials and hydrolysis processes, were collected over a period of 636 hours. The experimental results showed that hydrolysis occurred at different times for the different raw materials and hydrolysis processes. Therefore, after characterizing the protein bands in gels (SDS-PAGE) and paper chromatography of silages produced with different raw materials, processing methods, and interruption methods, protein fractionation differed with increasing hydrolysis time. Silage production was viable and presented favorable nutritional characteristics for incorporation into fish diets.
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Copyright (c) 2025 Ligia Maria Neira, Luiz Flávio José dos Santos, Adriano Marques Gonçalves, Joao Martins Pizauro Junior, Dalton José Carneiro (Autor)
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